Team:UNICAMP-Brazil/Notebooks/September 14
From 2009.igem.org
(→Culture growth of bacteria containing the F plasmid) |
(→PY Promoter - Purification of the digestion reactions) |
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*<p style=”text-align:justify;”>We purified the digestion reactions of PY1 and PY2 with the Invitrogen's PureLink™ PCR Purification Kit, following the manufacturer's protocol without modifications.</p> | *<p style=”text-align:justify;”>We purified the digestion reactions of PY1 and PY2 with the Invitrogen's PureLink™ PCR Purification Kit, following the manufacturer's protocol without modifications.</p> | ||
- | *<p style=”text-align:justify;”>We also prepared an agarose gel to run the digestion reaction of BBa_J23100. The band in the gel | + | *<p style=”text-align:justify;”>We also prepared an agarose gel to run the digestion reaction of BBa_J23100. The band in the gel had the expected size (2940 bp). However, it is not possible to confirm the digestion by the size in the gel because the difference between the plasmid BBa_J23100 with and without the fragment excised by ''Xba''I and ''Spe''I is too small.</p> |
[[Image:py_gel_2.png|200px|center]] | [[Image:py_gel_2.png|200px|center]] |
Latest revision as of 02:35, 22 October 2009
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