Team:Cambridge

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<h1>Overview</h1>
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Previous iGEM teams have focused on genetically engineering bacteria to respond to novel inputs – for example light, or biologically significant compounds. There is an unmistakable need, therefore, to also develop clear, user-friendly outputs, especially for use with biosensors. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy.  However, how much easier would it be if we could simply ''see'' the output with our own eyes? 
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'''The Cambridge [https://2009.igem.org/Main_Page 2009 iGEM] team has created two kits of parts that will facilitate the design and construction of biosensors in the the future.
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The Cambridge 2008 iGEM team is engineering E. coli to produce different pigments in response to different concentrations of an inducer. Our device is a three part system:
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Previous iGEM teams have focused on genetically engineering bacterial biosensors by enabling bacteria to respond to novel inputs, especially biologically significant compounds. There is an unmistakable need to also develop devices that can '''1) manipulate input by changing the behaviour of the response of the input-sensitive promoter''', and that can '''2) report a response using clear, user-friendly outputs'''. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy. But, what if we could simply see the output with our own eyes?
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We successfully characterised a set of transcriptional systems for calibrated output - [https://2009.igem.org/Team:Cambridge/Project/Amplification Sensitivity Tuners].  We also successfully expressed a spectrum of pigments in ''E. coli,'' designing a set of [https://2009.igem.org/Team:Cambridge/Project/Pigments Colour Generators].
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The Cambridge 2009 iGEM team is engineering E. coli to produce a range of pigments in order to equip future projects with better, more reliable, discrete outputs under logic control. Further, our bacteria utilize an amplifying mechanism, which effectively acts as an "on" switch to guarantee maximum pigment production even with low level input. This amplification part of the project aims to debug and improve the system created by the Cambridge 2007 team.
 
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<div class="imagelink_1">[https://2009.igem.org/Team:Cambridge/Project]</div><div class="imagelink_2">[https://2009.igem.org/Team:Cambridge/Project/Amplification]</div><div class="imagelink_3">[https://2009.igem.org/Team:Cambridge/Project/Pigments]</div><div class="imagelink_4">[https://2009.igem.org/Team:Cambridge/ImageGallery/TEAM]</div><div class="imagelink_5">[https://2009.igem.org/Team:Cambridge/Team]</div><div class="imagelink_6">[https://2009.igem.org/Team:Cambridge/ImageGallery]</div>
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==== The E. Chromi Team, in Cambridge's 800th year ====
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[[Image:Cambridgeteamphoto.png | 360px]][[Image:IGEMTSHIRTtrans2.png | 360px]]
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Latest revision as of 00:10, 22 October 2009


E. Chromi

Cambridge Frontpage2.png
RAINBOW.png

The Cambridge 2009 iGEM team has created two kits of parts that will facilitate the design and construction of biosensors in the the future.

Previous iGEM teams have focused on genetically engineering bacterial biosensors by enabling bacteria to respond to novel inputs, especially biologically significant compounds. There is an unmistakable need to also develop devices that can 1) manipulate input by changing the behaviour of the response of the input-sensitive promoter, and that can 2) report a response using clear, user-friendly outputs. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy. But, what if we could simply see the output with our own eyes?

We successfully characterised a set of transcriptional systems for calibrated output - Sensitivity Tuners. We also successfully expressed a spectrum of pigments in E. coli, designing a set of Colour Generators.















[1]
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The E. Chromi Team, in Cambridge's 800th year

Cambridgeteamphoto.pngIGEMTSHIRTtrans2.png

Cambridge Sponsor Logo1.pngCambridge Sponsor Logo2.pngCambridge Sponsor Logo3.pngCambridge Sponsor Logo4.pngCambridge Sponsor Logo5.pngCambridge Sponsor Logo8.pngCambridge Sponsor Logo6.pngCambridge Sponsor Logo7.pngCambridge Sponsor Logo9.pngCambridge Sponsor Logo10.pngCambridge Sponsor Logo11.pngCambridge Sponsor Logo12.pngCambridge Sponsor Logo14.pngCambridge Sponsor Logo13.pngCambridge Sponsor Logo15.pngCambridge Sponsor Logo16.pngCambridge Sponsor Logo17.pngCambridge Sponsor Logo18.pngCambridge Sponsor Logo19.pngBmglab.jpg

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