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Team:UNICAMP-Brazil/Notebooks/August 7
From 2009.igem.org
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| + | __NOTOC__ | ||
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| + | ==''' ColiGuard '''== | ||
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| + | ====Genomic DNA Extraction for Hly Operon Manipulation==== | ||
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| + | *<p style=”text-align:justify;”>We performed the genomic DNA Extraction from four pathogenic ''E. coli'' hly+ strains. We used the Aubusel 1998 protocol with modifications, [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/DNA_extraction Protocol 1].</p> | ||
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| + | ''Marcos'' | ||
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| + | ==''' YeastGuard '''== | ||
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| + | ====Recovering the biobricks==== | ||
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| + | *<p style=”text-align:justify;”>5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were replated into two diferent antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies.</p> | ||
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| + | *<p style=”text-align:justify;”>We inoculated some of the grown colonies of each biobrick in liquid media to perform miniprep tomorrow.</p> | ||
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| + | ''Raíssa and Wesley'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 00:14, 22 October 2009
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