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Team:UNICAMP-Brazil/Notebooks/July 20
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| - | During the week from July 20th to 25th we designed all primers that we´re going to use in our | + | {{:Team:UNICAMP-Brazil/inc_topo}} |
| + | {{:Team:UNICAMP-Brazil/inc calendar}} | ||
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| + | __NOTOC__ | ||
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| + | ==''' MicroGuards '''== | ||
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| + | ====Primer design==== | ||
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| + | * During the week from July 20th to 25th we designed all primers that we´re going to use in our project. All the sequences are showed on July 25th notebook. | ||
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| + | ''Everybody'' | ||
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| + | ==''' ColiGuard '''== | ||
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| + | ==== Restriction Assay==== | ||
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| + | * After the recovery of F plasmid (July 16th), we performed a digestion of the miniprep's product, in order to assure that we indeed recover the plasmid itself. We did this assay by using two restriction enzymes: ''EcoR''I and ''Cla''I. | ||
| + | * We then compared the pattern provided us by pDRAW software with an electrophoresis gel of the digestion product. According to this assay, we indeed were able to recover the F Plasmid! =] | ||
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| + | [[image:Digestao_EcoRI_ClaI-20-07-09.jpg|center]] | ||
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| + | ''Gabriel, Marcelo and Victor.'' | ||
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{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 00:04, 22 October 2009
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