Team:UNICAMP-Brazil/Notebooks/August 9

From 2009.igem.org

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{{:Team:UNICAMP-Brazil/inc calendar}}
{{:Team:UNICAMP-Brazil/inc calendar}}
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==Coliguard: selection of BioBricks==
+
__NOTOC__
 +
 
 +
==''' ColiGuard '''==
 +
 
 +
====Selection of BioBricks====
Today we selected the biobricks that we are going to use in the Coliguard subproject.
Today we selected the biobricks that we are going to use in the Coliguard subproject.
They are listed below.
They are listed below.
-
'''Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss'''   
+
*'''Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss'''   
Spring 2009 Distribution>2009 Kit Plate 1>1H
Spring 2009 Distribution>2009 Kit Plate 1>1H
Line 13: Line 17:
Spring 2008 Distribution>Source Plate 1002>5G
Spring 2008 Distribution>Source Plate 1002>5G
-
'''Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030'''
+
*'''Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030'''
   
   
Spring 2009 Distribution>2009 Kit Plate 1>2I
Spring 2009 Distribution>2009 Kit Plate 1>2I
Line 19: Line 23:
Spring 2008 Distribution>Source Plate 1000>2C
Spring 2008 Distribution>Source Plate 1000>2C
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'''Part:BBa_J61100 -  Ribosome Binding Site Family Member'''  
+
*'''Part:BBa_J61100 -  Ribosome Binding Site Family Member'''  
   
   
Spring 2009 Distribution>2009 Kit Plate 1>5J
Spring 2009 Distribution>2009 Kit Plate 1>5J
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Spring 2008 Distribution>Source Plate 1003>11F
Spring 2008 Distribution>Source Plate 1003>11F
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'''Part:BBa_J61107 - Ribosome Binding Site Family Member'''  
+
*'''Part:BBa_J61107 - Ribosome Binding Site Family Member'''  
Spring 2009 Distribution>2009 Kit Plate 1>5N
Spring 2009 Distribution>2009 Kit Plate 1>5N
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Spring 2008 Distribution>Source Plate 1003>12E
Spring 2008 Distribution>Source Plate 1003>12E
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'''Part:BBa_B0010 - T1 from E. coli rrnB'''  
+
*'''Part:BBa_B0010 - T1 from E. coli rrnB'''  
Spring 2009 Distribution>2009 Kit Plate 1>13D
Spring 2009 Distribution>2009 Kit Plate 1>13D
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Spring 2008 Distribution>Source Plate 1004>5D
Spring 2008 Distribution>Source Plate 1004>5D
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'''Part:BBa_B0014 - double terminator'''  
+
*'''Part:BBa_B0014 - double terminator'''  
Spring 2009 Distribution>2009 Kit Plate 2>24C
Spring 2009 Distribution>2009 Kit Plate 2>24C
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Spring 2008 Distribution>Source Plate 1016>2E
Spring 2008 Distribution>Source Plate 1016>2E
-
'''Part:BBa_B0015 - double terminator (B0010-B0012) '''  
+
*'''Part:BBa_B0015 - double terminator (B0010-B0012) '''  
Spring 2009 Distribution>2009 Kit Plate 1>23L
Spring 2009 Distribution>2009 Kit Plate 1>23L
Line 49: Line 53:
Spring 2008 Distribution>Source Plate 1016>5F
Spring 2008 Distribution>Source Plate 1016>5F
-
'''Part:BBa_R0040 -  TetR repressible promoter'''  
+
*'''Part:BBa_R0040 -  TetR repressible promoter'''  
Spring 2009 Distribution>2009 Kit Plate 1>6I
Spring 2009 Distribution>2009 Kit Plate 1>6I
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Spring 2008 Distribution>Source Plate 1000>4C
Spring 2008 Distribution>Source Plate 1000>4C
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'''Part:BBa_J23100 - constitutive promoter family member'''  
+
*'''Part:BBa_J23100 - constitutive promoter family member'''  
Spring 2009 Distribution>2009 Kit Plate 1>18C
Spring 2009 Distribution>2009 Kit Plate 1>18C
Line 61: Line 65:
Spring 2008 Distribution>Source Plate 1002>10E
Spring 2008 Distribution>Source Plate 1002>10E
-
'''Part:BBa_J23107 - constitutive promoter family member'''  
+
*'''Part:BBa_J23107 - constitutive promoter family member'''  
Spring 2009 Distribution>2009 Kit Plate 1>20A
Spring 2009 Distribution>2009 Kit Plate 1>20A
Line 67: Line 71:
Spring 2008 Distribution>Source Plate 1002>11D
Spring 2008 Distribution>Source Plate 1002>11D
-
'''Part:BBa_J23109 - constitutive promoter family member'''  
+
*'''Part:BBa_J23109 - constitutive promoter family member'''  
Spring 2009 Distribution>2009 Kit Plate 2>2G
Spring 2009 Distribution>2009 Kit Plate 2>2G
Line 73: Line 77:
Spring 2009 Source Plates>S09 SP 2005>1D
Spring 2009 Source Plates>S09 SP 2005>1D
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'''Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)'''   
+
*'''Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)'''   
Spring 2009 Distribution>2009 Kit Plate 1>14K
Spring 2009 Distribution>2009 Kit Plate 1>14K
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Spring 2008 Distribution>Source Plate 1001>7E
Spring 2008 Distribution>Source Plate 1001>7E
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'''Part:BBa_J31005:Get Part - chloramphenicol acetyltransferase (forwards, CmF)'''  
+
*'''Part:BBa_J31005:Get Part - chloramphenicol acetyltransferase (forwards, CmF)'''  
Spring 2009 Distribution>2009 Kit Plate 1>1P
Spring 2009 Distribution>2009 Kit Plate 1>1P
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Spring 2008 Distribution>Source Plate 1002>6C
Spring 2008 Distribution>Source Plate 1002>6C
-
'''Part:BBa_J31007 - tetracycline resistance protein TetA(C)'''  
+
*'''Part:BBa_J31007 - tetracycline resistance protein TetA(C)'''  
Spring 2009 Distribution>2009 Kit Plate 1>1L
Spring 2009 Distribution>2009 Kit Plate 1>1L
Line 91: Line 95:
Spring 2008 Distribution>Source Plate 1002>6ª
Spring 2008 Distribution>Source Plate 1002>6ª
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'''Part:BBa_J31003 - kanamycin resistance forward'''  
+
*'''Part:BBa_J31003 - kanamycin resistance forward'''  
Spring 2008 Distribution>Source Plate 1002>6E
Spring 2008 Distribution>Source Plate 1002>6E
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Registry 2006>2006 Source Plate 1>3E
Registry 2006>2006 Source Plate 1>3E
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'''Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata  
+
*'''Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata  
'''
'''
Line 104: Line 108:
Spring 2008 Distribution>Source Plate 1013>7G
Spring 2008 Distribution>Source Plate 1013>7G
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'''Part:pSB1A3 -  High copy BioBrick assembly plasmid'''  
+
*'''Part:pSB1A3 -  High copy BioBrick assembly plasmid'''  
Spring 2009 Distribution 2009 Kit Plate 1 1K
Spring 2009 Distribution 2009 Kit Plate 1 1K
-
'''Part:pSB1AK3 - High copy BioBrick assembly plasmid'''  
+
*'''Part:pSB1AK3 - High copy BioBrick assembly plasmid'''  
Spring 2009 Distribution2009 Kit Plate 1 13A
Spring 2009 Distribution2009 Kit Plate 1 13A
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'''Part:pSB3K3 -  Low to medium copy BioBrick standard vector'''  
+
*'''Part:pSB3K3 -  Low to medium copy BioBrick standard vector'''  
Spring 2009 Distribution2009 Kit Plate  17E
Spring 2009 Distribution2009 Kit Plate  17E
-
'''Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)'''  
+
*'''Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)'''  
Spring 2009 Distribution2009 Kit Plate  1    1O  BBa_J04450
Spring 2009 Distribution2009 Kit Plate  1    1O  BBa_J04450

Latest revision as of 20:29, 10 October 2009

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ColiGuard

Selection of BioBricks

Today we selected the biobricks that we are going to use in the Coliguard subproject. They are listed below.

  • Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss

Spring 2009 Distribution>2009 Kit Plate 1>1H

Spring 2008 Distribution>Source Plate 1002>5G

  • Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030

Spring 2009 Distribution>2009 Kit Plate 1>2I

Spring 2008 Distribution>Source Plate 1000>2C

  • Part:BBa_J61100 - Ribosome Binding Site Family Member

Spring 2009 Distribution>2009 Kit Plate 1>5J

Spring 2008 Distribution>Source Plate 1003>11F

  • Part:BBa_J61107 - Ribosome Binding Site Family Member

Spring 2009 Distribution>2009 Kit Plate 1>5N

Spring 2008 Distribution>Source Plate 1003>12E

  • Part:BBa_B0010 - T1 from E. coli rrnB

Spring 2009 Distribution>2009 Kit Plate 1>13D

Spring 2008 Distribution>Source Plate 1004>5D

  • Part:BBa_B0014 - double terminator

Spring 2009 Distribution>2009 Kit Plate 2>24C

Spring 2008 Distribution>Source Plate 1016>2E

  • Part:BBa_B0015 - double terminator (B0010-B0012)

Spring 2009 Distribution>2009 Kit Plate 1>23L

Spring 2008 Distribution>Source Plate 1016>5F

  • Part:BBa_R0040 - TetR repressible promoter

Spring 2009 Distribution>2009 Kit Plate 1>6I

Spring 2008 Distribution>Source Plate 1000>4C

  • Part:BBa_J23100 - constitutive promoter family member

Spring 2009 Distribution>2009 Kit Plate 1>18C

Spring 2008 Distribution>Source Plate 1002>10E

  • Part:BBa_J23107 - constitutive promoter family member

Spring 2009 Distribution>2009 Kit Plate 1>20A

Spring 2008 Distribution>Source Plate 1002>11D

  • Part:BBa_J23109 - constitutive promoter family member

Spring 2009 Distribution>2009 Kit Plate 2>2G

Spring 2009 Source Plates>S09 SP 2005>1D

  • Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)

Spring 2009 Distribution>2009 Kit Plate 1>14K

Spring 2008 Distribution>Source Plate 1001>7E

  • Part:BBa_J31005:Get Part - chloramphenicol acetyltransferase (forwards, CmF)

Spring 2009 Distribution>2009 Kit Plate 1>1P

Spring 2008 Distribution>Source Plate 1002>6C

  • Part:BBa_J31007 - tetracycline resistance protein TetA(C)

Spring 2009 Distribution>2009 Kit Plate 1>1L

Spring 2008 Distribution>Source Plate 1002>6ª

  • Part:BBa_J31003 - kanamycin resistance forward

Spring 2008 Distribution>Source Plate 1002>6E

Registry 2006>2006 Source Plate 1>3E

  • Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata

Spring 2009 Distribution>2009 Kit Plate 1>18F

Spring 2008 Distribution>Source Plate 1013>7G

  • Part:pSB1A3 - High copy BioBrick assembly plasmid

Spring 2009 Distribution 2009 Kit Plate 1 1K

  • Part:pSB1AK3 - High copy BioBrick assembly plasmid

Spring 2009 Distribution2009 Kit Plate 1 13A

  • Part:pSB3K3 - Low to medium copy BioBrick standard vector

Spring 2009 Distribution2009 Kit Plate 17E

  • Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)

Spring 2009 Distribution2009 Kit Plate 1 1O BBa_J04450