Team:UNICAMP-Brazil/Notebooks/July 24
From 2009.igem.org
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+ | __NOTOC__ | ||
- | == | + | ==''' ColiGuard '''== |
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+ | ==== R Plasmid Minipreps ==== | ||
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+ | * Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process). | ||
+ | * We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. | ||
+ | * We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control. | ||
+ | * As we did for F plasmid, we were also able to recover the R plasmid. | ||
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+ | [[image:Teste_plasmideos_10072009.jpg|center]] | ||
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+ | ''Gabriel, Marcelo and Victor.'' | ||
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{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Latest revision as of 19:47, 19 October 2009
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