Team:UNICAMP-Brazil/Notebooks/August 5

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__NOTOC__
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==Preparation of electrocompetent ''E. coli''==
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==''' MicroGuards '''==
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Today we prepared electrocompetent ''E. coli'' to use in our transformations according to protocol 4 (see protocol section).
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====Preparation of electrocompetent ''E. coli''====
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'''Marcelo and Luige'''
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* Today we prepared electrocompetent ''E. coli'' to use in our transformations according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Preparation_of_electrocompetent_E._coli Protocol 4].
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==Yeast DNA extraction==
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''Marcelo and Luige''
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Today we performed DNA extration from yeast ''Kluyveromyces lactis'' according to protocol 5(see protocol section). The agarose gel showed that the mini-prep was successful, but we need to treat the DNA with RNAse.
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==''' YeastGuard '''==
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==== Yeast DNA extraction ====
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* Today we performed DNA extration from yeast ''Kluyveromyces lactis'' according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Yeast_DNA_Extraction Protocol 5]. The extraction was successful.
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Latest revision as of 05:30, 21 October 2009

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MicroGuards

Preparation of electrocompetent E. coli

  • Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4.

Marcelo and Luige

YeastGuard

Yeast DNA extraction

  • Today we performed DNA extration from yeast Kluyveromyces lactis according to Protocol 5. The extraction was successful.




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