Team:UNICAMP-Brazil/Notebooks/July 31

YeastGuard

Selection of BioBricks

Today we selected the biobricks that we are going to use in the Yeastguard subproject. They are listed below.

Part:BBa_J63005 - yeast promoter -- ADH1 promoter from S. cerevisiae

Spring 2009 Distribution>2009 Kit Plate 2>4A

Spring 2008 Distribution>Source Plate 1009>2C

Part:BBa_J63003 - yeast RBS -- designed yeast Kozak sequence

Spring 2009 Distribution>2009 Kit Plate 2>2O

Spring 2008 Distribution>Source Plate 1003>3B

Part:BBa_J63002 - yeast terminator -- ADH1 terminator from S. cerevisiae

Spring 2008 Distribution>Source Plate 1003>2G

Spring 2007 Distribution>iGEM 2007 Parts Kit Plate 3>8I

Part:BBa_E2030 - yellow reporter -- enhanced version of EYFP, yeast-optimized

Spring 2009 Distribution>2009 Kit Plate 1>12D

Spring 2008 Distribution>Source Plate 1013>10D

Part:BBa_E2020 - cyan reporter -- enhanced version of ECFP, yeast-optimized

Spring 2009 Distribution>2009 Kit Plate 1>12B

Spring 2008 Distribution>Source Plate 1013>10C

Part:BBa_J04630 - GFP reporter

Spring 2009 Distribution>2009 Kit Plate 3>19F

Spring 2008 Distribution>Source Plate 1016>10E

Part:BBa_J63010 - plasmid with BBa_J04450 -- Protein fusion vector (Silver lab standard)

Spring 2009 Distribution>2009 Kit Plate 1>1G