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Team:Cambridge/Project/Carotenoids/PR
From 2009.igem.org
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| - | [ | + | [https://2009.igem.org/Team:Cambridge/Project/Carotenoids/Int Introduction] |
| - | [ | + | [https://2009.igem.org/Team:Cambridge/Project/Carotenoids/PR Previous Research] |
| - | [ | + | [https://2009.igem.org/Team:Cambridge/Project/Carotenoids/OAP Our Action Plan] |
| - | [ | + | [https://2009.igem.org/Team:Cambridge/Project/Carotenoids/RA Result Analysis] |
| - | [ | + | [https://2009.igem.org/Team:Cambridge/Project/Carotenoids/Ref Reference] |
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! Sequence Description | ! Sequence Description | ||
! Size | ! Size | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#FFFFFF;" cellpadding="6" cellspacing="0" border="1" |
|K118014 | |K118014 | ||
|Edinburgh 08 | |Edinburgh 08 | ||
| - | |rbs + CrtE | + | |[[Image:Icon translational unit.png]] (rbs + CrtE) |
|insert: 922bp, backbone: 2079bp, total: 3001bp. | |insert: 922bp, backbone: 2079bp, total: 3001bp. | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#FFFFFF;" cellpadding="6" cellspacing="0" border="1" |
|K118006 | |K118006 | ||
|Edinburgh 08 | |Edinburgh 08 | ||
| - | |rbs + CrtB | + | |[[Image:Icon translational unit.png]] (rbs + CrtB) |
|insert: 949bp, backbone: 2079bp, total: 3028bp. | |insert: 949bp, backbone: 2079bp, total: 3028bp. | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#FFFFFF;" cellpadding="6" cellspacing="0" border="1" |
|K118005 | |K118005 | ||
|Edinburgh 08 | |Edinburgh 08 | ||
| - | |rbs + CrtI | + | |[[Image:Icon translational unit.png]] (rbs + CrtI) |
|insert: 1498bp, backbone: 2079bp, total: 3577bp. | |insert: 1498bp, backbone: 2079bp, total: 3577bp. | ||
2 PstI sites removed. | 2 PstI sites removed. | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#FFFFFF;" cellpadding="6" cellspacing="0" border="1" |
|K118013 | |K118013 | ||
|Edinburgh 08 | |Edinburgh 08 | ||
| - | |rbs + CrtY | + | |[[Image:Icon translational unit.png]] (rbs + CrtY) |
|insert: 1162bp, backbone: 2079bp, total: 3241bp. | |insert: 1162bp, backbone: 2079bp, total: 3241bp. | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#FFFFFF;" cellpadding="6" cellspacing="0" border="1" |
|K152005 | |K152005 | ||
|Guelph 08 | |Guelph 08 | ||
| - | |(rbs+CrtE) + (rbs+CrtB) + (rbs+CrtI) | + | |[[Image:Icon translational unit.png]][[Image:Icon translational unit.png]][[Image:Icon translational unit.png]][[Image:Icon translational unit.png]][[Image:Icon reporter.png]] |
| + | (rbs+CrtE) + (rbs+CrtB) + (rbs+CrtI) | ||
+ (rbs+CrtY) + (rbs+GFP) | + (rbs+CrtY) + (rbs+GFP) | ||
|insert: 5412bp, backbone: 2079bp, total: 7491bp. | |insert: 5412bp, backbone: 2079bp, total: 7491bp. | ||
2 PstI sites in CrtI removed. | 2 PstI sites in CrtI removed. | ||
| - | |- style="color:#333; background-color:# | + | |- style="color:#333; background-color:#FFFFFF;" cellpadding="6" cellspacing="0" border="1" |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
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|} | |} | ||
| - | |||
| + | The above enzymes all come from a type of Enterobacteriaceae bacteria, '''''Pantoea ananatis''''' (GenBank: D90087.2). | ||
| - | + | ||
| + | During our preliminary research, we have encountered a few issues: | ||
* In part K152005, the “CrtE” sequence (which originated from part K152001) contains 3 nucleotides different from the NCBI data base. | * In part K152005, the “CrtE” sequence (which originated from part K152001) contains 3 nucleotides different from the NCBI data base. | ||
| - | * Originally, the registry annotation for K152005 indicated that there was no ribosome binding site before gene CrtI (i.e. “…rbs+CrtB+CrtI+rbs+CrtY…”). After clarification with Team Guelph 08, we were told that it was | + | |
| - | * Teams of previous years constructed various composite parts containing two or more enzymes of the synthetic pathways (e.g. CrtEBI, CrtEBIY), but they | + | * Originally, the registry annotation for K152005 indicated that there was no ribosome binding site before gene CrtI (i.e. “…rbs+CrtB+CrtI+rbs+CrtY…”). After clarification with Team Guelph 08, we were told that it was an error in annotation. The registry entry for K152005 has since been updated to include the ribosome binding site. |
| + | |||
| + | * Teams of previous years constructed various composite parts containing two or more enzymes of the synthetic pathways (e.g. CrtEBI, CrtEBIY), but they are not available on the 2009 Distribution Plates. | ||
<!--Do not remove the first and last lines in this page!-->{{Template:CambridgeBottom}} | <!--Do not remove the first and last lines in this page!-->{{Template:CambridgeBottom}} | ||
Latest revision as of 13:53, 2 September 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Carotenoids
Previous Research
A few iGEM teams of previous years (e.g. Edinburgh 07, Edinburgh 08, Guelph 08) conducted research in the carotenoid system. We are fortunate to have access to some of their Biobricks in the registry. Here is a summary of the Biobricks available:
| Registry Code | Team | Sequence Description | Size |
|---|---|---|---|
| K118014 | Edinburgh 08 |  (rbs + CrtE)
| insert: 922bp, backbone: 2079bp, total: 3001bp. |
| K118006 | Edinburgh 08 | (rbs + CrtB)
| insert: 949bp, backbone: 2079bp, total: 3028bp. |
| K118005 | Edinburgh 08 | (rbs + CrtI)
| insert: 1498bp, backbone: 2079bp, total: 3577bp.
2 PstI sites removed. |
| K118013 | Edinburgh 08 | (rbs + CrtY)
| insert: 1162bp, backbone: 2079bp, total: 3241bp. |
| K152005 | Guelph 08 | 
(rbs+CrtE) + (rbs+CrtB) + (rbs+CrtI) + (rbs+CrtY) + (rbs+GFP) | insert: 5412bp, backbone: 2079bp, total: 7491bp.
2 PstI sites in CrtI removed. |
The above enzymes all come from a type of Enterobacteriaceae bacteria, Pantoea ananatis (GenBank: D90087.2).
During our preliminary research, we have encountered a few issues:
- In part K152005, the “CrtE” sequence (which originated from part K152001) contains 3 nucleotides different from the NCBI data base.
- Originally, the registry annotation for K152005 indicated that there was no ribosome binding site before gene CrtI (i.e. “…rbs+CrtB+CrtI+rbs+CrtY…”). After clarification with Team Guelph 08, we were told that it was an error in annotation. The registry entry for K152005 has since been updated to include the ribosome binding site.
- Teams of previous years constructed various composite parts containing two or more enzymes of the synthetic pathways (e.g. CrtEBI, CrtEBIY), but they are not available on the 2009 Distribution Plates.
