Team:UNICAMP-Brazil/Notebooks/August 15

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== YeastGuard: RBS and CFP plasmid digestion ==
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{{:Team:UNICAMP-Brazil/inc_topo}}
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{{:Team:UNICAMP-Brazil/inc calendar}}
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- Today we started the construction of our first biobrick. After quantificate the mini-prep's products we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.
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__NOTOC__
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- After the digestion we isolated the target bands from the agarose gel, according to the protocol X.
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==''' YeastGuard '''==
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- The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.
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====Biobricks recovered: RBS and CFP digestion====
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'''Raíssa'''
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*<p style=”text-align:justify;”>Today we started the construction of our first biobrick. After quantification of the miniprep products (from August 11) we performed the digestion ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]) with ''Spe''I/''Pst''I and ''Xba''I/''Pst''I of plasmids containing the RBS and the CFP reporter respectively in order to connect these parts.</p>
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*<p style=”text-align:justify;”>After the digestion we isolated the target bands from the agarose gel, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7].</p>
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[[image:20090815_Digestões-Lig 1.jpg|500px|center]]
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*<p style=”text-align:justify;”>The CFP digested band was correctly purified from the gel. Unfortunatly, we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate these two parts today.</p>
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''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 00:51, 22 October 2009

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YeastGuard

Biobricks recovered: RBS and CFP digestion

  • Today we started the construction of our first biobrick. After quantification of the miniprep products (from August 11) we performed the digestion (Protocol 14) with SpeI/PstI and XbaI/PstI of plasmids containing the RBS and the CFP reporter respectively in order to connect these parts.

  • After the digestion we isolated the target bands from the agarose gel, according to Protocol 7.

20090815 Digestões-Lig 1.jpg

  • The CFP digested band was correctly purified from the gel. Unfortunatly, we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate these two parts today.

Raíssa




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