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Team:UNICAMP-Brazil/Notebooks/July 20
From 2009.igem.org
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{{:Team:UNICAMP-Brazil/inc calendar}} | {{:Team:UNICAMP-Brazil/inc calendar}} | ||
| - | + | __NOTOC__ | |
| - | + | ==''' MicroGuards '''== | |
| - | == | + | ====Primer design==== |
| - | After the recovery of F plasmid (July 16th), we performed a digestion of the | + | * During the week from July 20th to 25th we designed all primers that we´re going to use in our project. All the sequences are showed on July 25th notebook. |
| - | We did this assay by using two restriction enzymes: | + | |
| - | We then compared the pattern provided us by pDRAW software with an | + | ''Everybody'' |
| - | According to this assay, we indeed were able to recover the F Plasmid! | + | |
| - | =] | + | ==''' ColiGuard '''== |
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| + | ==== Restriction Assay==== | ||
| + | |||
| + | * After the recovery of F plasmid (July 16th), we performed a digestion of the miniprep's product, in order to assure that we indeed recover the plasmid itself. We did this assay by using two restriction enzymes: ''EcoR''I and ''Cla''I. | ||
| + | * We then compared the pattern provided us by pDRAW software with an electrophoresis gel of the digestion product. According to this assay, we indeed were able to recover the F Plasmid! =] | ||
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| + | [[image:Digestao_EcoRI_ClaI-20-07-09.jpg|center]] | ||
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| + | ''Gabriel, Marcelo and Victor.'' | ||
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{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 00:04, 22 October 2009
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