Team:UNICAMP-Brazil/Notebooks/July 24

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{{:Team:UNICAMP-Brazil/inc calendar}}
{{:Team:UNICAMP-Brazil/inc calendar}}
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__NOTOC__
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==Primer design==
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==''' ColiGuard '''==
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During the week from July 20th to 25th we designed all primers that we´re going to use in our ptoject. All the sequences are showed on July 25th notebook.
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==== R Plasmid Minipreps ====
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==Coliguard: R Plasmid Mini-Preps==
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* Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
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* We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].
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* We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control.
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* As we did for F plasmid, we were also able to recover the R plasmid.
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Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
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[[image:Teste_plasmideos_10072009.jpg|center]]
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We performed mini-preps from R plasmid-containing cultures, according to protocol nº2 (see protocols section).
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As we did for F plasmid, we were also able to recover the R plasmid.
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'''Gabriel, Marcelo and Victor.'''
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''Gabriel, Marcelo and Victor.''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 19:47, 19 October 2009

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ColiGuard

R Plasmid Minipreps

  • Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
  • We performed minipreps from R plasmid-containing cultures, according to Protocol 2.
  • We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control.
  • As we did for F plasmid, we were also able to recover the R plasmid.
Teste plasmideos 10072009.jpg


Gabriel, Marcelo and Victor.





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