Team:UNICAMP-Brazil/Notebooks/July 24

(Difference between revisions)

Latest revision as of 19:47, 19 October 2009

Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
We performed minipreps from R plasmid-containing cultures, according to Protocol 2.
We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control.
As we did for F plasmid, we were also able to recover the R plasmid.

Gabriel, Marcelo and Victor.

@@ Line 6: / Line 6: @@
 ==''' ColiGuard '''==
-==== R Plasmid Mini-Preps ====
+==== R Plasmid Minipreps ====
+* Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
+* We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].
+* We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control.
+* As we did for F plasmid, we were also able to recover the R plasmid.
+[[image:Teste_plasmideos_10072009.jpg|center]]
-Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
-We performed mini-preps from R plasmid-containing cultures, according to Protocol 2 (see protocols section).
-As we did for F plasmid, we were also able to recover the R plasmid.
 ''Gabriel, Marcelo and Victor.''