Team:UNICAMP-Brazil/Notebooks/August 5

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====Preparation of electrocompetent ''E. coli''====
====Preparation of electrocompetent ''E. coli''====
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* Today we prepared electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 (see protocols section).
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* Today we prepared electrocompetent ''E. coli'' to use in our transformations according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Preparation_of_electrocompetent_E._coli Protocol 4].
''Marcelo and Luige''
''Marcelo and Luige''
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==== Yeast DNA extraction ====
==== Yeast DNA extraction ====
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* Today we performed DNA extration from yeast ''Kluyveromyces lactis'' according to Protocol 5 (see protocols section). The agarose gel showed that the mini-prep was successful, but we need to treat the DNA with RNAse.
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* Today we performed DNA extration from yeast ''Kluyveromyces lactis'' according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Yeast_DNA_Extraction Protocol 5]. The extraction was successful.
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 05:30, 21 October 2009

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MicroGuards

Preparation of electrocompetent E. coli

  • Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4.

Marcelo and Luige

YeastGuard

Yeast DNA extraction

  • Today we performed DNA extration from yeast Kluyveromyces lactis according to Protocol 5. The extraction was successful.




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