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Team:UNICAMP-Brazil/Notebooks/July 24
From 2009.igem.org
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==== R Plasmid Minipreps ==== | ==== R Plasmid Minipreps ==== | ||
| - | Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process). | + | * Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process). |
| - | We performed minipreps from R plasmid-containing cultures, according to Protocol 2 | + | * We performed minipreps from R plasmid-containing cultures, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. |
| - | As we did for F plasmid, we were also able to recover the R plasmid. | + | * We then ran an agarose gel with miniprep's sample, also running a few F plasmid's sample as a band-size control. |
| + | * As we did for F plasmid, we were also able to recover the R plasmid. | ||
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| + | [[image:Teste_plasmideos_10072009.jpg|center]] | ||
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''Gabriel, Marcelo and Victor.'' | ''Gabriel, Marcelo and Victor.'' | ||
Latest revision as of 19:47, 19 October 2009
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