Team:UNICAMP-Brazil/Notebooks/August 29

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=====Material=====
=====Material=====
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We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of ''Gallus gallus'': the blood, the spleen and the oviduct.
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*<p style=”text-align:justify;”>We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of ''Gallus gallus'': the blood, the spleen and the oviduct. =)</p>
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[[Image:chicken_run_4.jpg|right]]
=====RNA extraction=====
=====RNA extraction=====
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We used the Trizol protocol for the three tissues (Protocol X).
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*<p style=”text-align:justify;”>We used the Trizol protocol for the three tissues ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/RNA_isolation_from_%27Gallus_gallus%27_spleen%2C_blood_and_oviduct Protocol 8]).</p>
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We obtained pellets from all tissues! Hope their concentration and quality are ok! =]
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*<p style=”text-align:justify;”>We obtained pellets from all tissues! Hope their concentration and quality are ok! =] </p>
   
   
''Gleidson, Raíssa, Taís and Sula''
''Gleidson, Raíssa, Taís and Sula''
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====Terminator Biobrick====
====Terminator Biobrick====
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Today we perfomed miniprep from the Terminator transformed ''E. coli'', according to Protocol 2 (see protocols section).
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*<p style=”text-align:justify;”>Today we perfomed miniprep of the Terminator Biobrick transformed ''E. coli'', according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].</p>
''Wesley''
''Wesley''
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====RBS+CFP====
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*<p style=”text-align:justify;”>Today we perfomed miniprep of the RBS+CFP Biobrick transformed ''E. coli'', according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].</p>
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''Raíssa and Taís''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 01:53, 22 October 2009

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YeastGuard

Lysozyme

Material

  • We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of Gallus gallus: the blood, the spleen and the oviduct. =)

Chicken run 4.jpg
RNA extraction

  • We used the Trizol protocol for the three tissues (Protocol 8).

  • We obtained pellets from all tissues! Hope their concentration and quality are ok! =]

Gleidson, Raíssa, Taís and Sula

Terminator Biobrick

  • Today we perfomed miniprep of the Terminator Biobrick transformed E. coli, according to Protocol 2.

Wesley

RBS+CFP

  • Today we perfomed miniprep of the RBS+CFP Biobrick transformed E. coli, according to Protocol 2.

Raíssa and Taís




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