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| </style></html> | | </style></html> |
| <div id="topicpath"> | | <div id="topicpath"> |
- | #[[#|Home]] | + | #[[Team:Kyoto|Home]] |
- | #[[#|GSDD]] | + | #[[Team:Kyoto/CiC|CiC]] |
| #Notebook | | #Notebook |
| + | {{:Team:Kyoto/topicpath}} |
| </div><!-- /#topicpath --> | | </div><!-- /#topicpath --> |
| {{:Team:Kyoto/Leftnavi}} | | {{:Team:Kyoto/Leftnavi}} |
- |
| |
| <!-- ** Edit below ** --> | | <!-- ** Edit below ** --> |
| <html> | | <html> |
- |
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- | <style>
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- | #calendar{
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- | width:100px;
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- | border:2px #CCC solid;
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- | background:#DDD;
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- | }
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- | #calendar ul{
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- | padding:0;
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- | margin:0;
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- | width:100px;
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- | list-style:none;
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- | }
| |
- | #calendar ul li{
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- | padding:0;
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- | margin:0;
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- | width:100px;
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- | height:30px;
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- | }
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- | #calendar ul li a{
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- | display:block;
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- | width:90px;
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- | height:30px;
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- | padding-left:10px;
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- | text-decoration:none;
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- | font-weight:bold;
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- | line-height:30px;
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- | }
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- |
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- | #calendar ul li a#calendar_week1{
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- | background:#EEE;
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- | }
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- |
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- | #calendar ul li a:link{
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- | }
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- | #calendar ul li a:hover{
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- | background:#EEE;
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- | }
| |
- | </style>
| |
| <div id="rightcontents"> | | <div id="rightcontents"> |
- | <div id="calendar">
| |
- | <ul>
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- | <li><a id="calendar_week1" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week1" title="1st week">1st week</a></li>
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- | <li><a id="calendar_week2" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week2" title="2nd week">2nd week</a></li>
| |
- | <li><a id="calendar_week3" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week3" title="3rd week">3rd week</a></li>
| |
- | <li><a id="calendar_week4" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week4" title="4th week">4th week</a></li>
| |
- | <li><a id="calendar_week5" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week5" title="5th week">5th week</a></li>
| |
- | <li><a id="calendar_week6" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week6" title="6th week">6th week</a></li>
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- | <li><a id="calendar_week7" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week7" title="7th week">7th week</a></li>
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- | <li><a id="calendar_week8" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week8" title="8th week">8th week</a></li>
| |
- | </ul>
| |
- | </div><!-- /#calendar -->
| |
| </html> | | </html> |
- | | + | {{:Team:Kyoto/CiC/Notebook_tpl}} |
- | ===Monday===
| + | <html> |
- | ====To Do on 24 July====
| + | </div><!-- /#rightcontents --></html> |
- | *Miniprep (3-O-2, 3-A-4)
| + | |
- | *Restriction Enzyme Digestion
| + | |
- | *Electrophoresis
| + | |
- | | + | |
- | ====Results====
| + | |
- | =====Miniprep=====
| + | |
- | *3-O-2, 3-A-4 was minipreped.
| + | |
- | | + | |
- | =====Digestion=====
| + | |
- | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| + | |
- | | + | |
- | {| border="1" style="text-align:center;width:50%" | + | |
- | |+Sample list
| + | |
- | !NO.!!Sample Name!!Enzyme
| + | |
- | |-align="center"
| + | |
- | |1||3-O-2||Eco R1, PST1
| + | |
- | |-align="center"
| + | |
- | |2||3-A-4||Eco R1, PST1
| + | |
- | |-align="center"
| + | |
- | |3||3-O-2||Eco R1
| + | |
- | |-align="center"
| + | |
- | |4||3-A-4||Eco R1
| + | |
- | |}
| + | |
- | | + | |
- | =====Electrophoresis=====
| + | |
- | [[Image:Kyoto_e_090724_1.png|250px]]
| + | |
- | * Very clear bands were found. (See the gel image in right!)
| + | |
- | * The bands are listed below.
| + | |
- | | + | |
- | {| border="1" style="text-align:center;width:50%" | + | |
- | |+Parts and Plasmid length
| + | |
- | !!!3-O-2!!3-A-4
| + | |
- | |-
| + | |
- | |Parts length / bp||2837||487
| + | |
- | |-
| + | |
- | |Plasmid length / bp||3266||3266
| + | |
- | |}
| + | |
- | <html></div><!-- /#rightcontents --></html> | + | |