Team:UNICAMP-Brazil/Notebooks/August 15

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==''' YeastGuard '''==
==''' YeastGuard '''==
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====RBS and CFP plasmid digestion====
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====Biobricks recovered: RBS and CFP digestion====
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* Today we started the construction of our first biobrick. After quantificate the miniprep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter.  
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*<p style=”text-align:justify;”>Today we started the construction of our first biobrick. After quantification of the miniprep products (from August 11) we performed the digestion ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]) with ''Spe''I/''Pst''I and ''Xba''I/''Pst''I of plasmids containing the RBS and the CFP reporter respectively in order to connect these parts.</p>
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* After the digestion we isolated the target bands from the agarose gel, according to the protocol 7.  
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*<p style=”text-align:justify;”>After the digestion we isolated the target bands from the agarose gel, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7].</p>
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* The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today.
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[[image:20090815_Digestões-Lig 1.jpg|500px|center]]
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*<p style=”text-align:justify;”>The CFP digested band was correctly purified from the gel. Unfortunatly, we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate these two parts today.</p>
''Raíssa''
''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

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YeastGuard

Biobricks recovered: RBS and CFP digestion

  • Today we started the construction of our first biobrick. After quantification of the miniprep products (from August 11) we performed the digestion (Protocol 14) with SpeI/PstI and XbaI/PstI of plasmids containing the RBS and the CFP reporter respectively in order to connect these parts.

  • After the digestion we isolated the target bands from the agarose gel, according to Protocol 7.

20090815 Digestões-Lig 1.jpg
  • The CFP digested band was correctly purified from the gel. Unfortunatly, we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate these two parts today.

Raíssa