Team:UNICAMP-Brazil/Notebooks/September 25

From 2009.igem.org

(Difference between revisions)
(→Getting the visa)
(→Biofusion vector - Electroelution)
 
(28 intermediate revisions not shown)
Line 1: Line 1:
-
==''' MicroGuards '''==
+
{{:Team:UNICAMP-Brazil/inc_topo}}
 +
{{:Team:UNICAMP-Brazil/inc calendar}}
 +
 
 +
__NOTOC__
 +
 
 +
==''' ColiGuard '''==
 +
 
 +
 
====Getting the visa====
====Getting the visa====
-
* Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D
+
*<p style=”text-align:justify;”>Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D</p>
-
*  
+
 
 +
====finO and finP - Still Trying to Confirm our Biobricks====
 +
 
 +
*<p style=”text-align:justify;”>We ran an agarose gel of yesterday's PCRs product.</p>
 +
*<p style=”text-align:justify;”>We couldn't obtain even a single amplified fragment! =(</p>
 +
*<p style=”text-align:justify;”>Why can the transformed cells grew in the media (that means they actually have the AMP resistence), but they haven't our inserts into it's plasmids?</p>
 +
*<p style=”text-align:justify;”>Our advisors suggested us that, since we digested our plasmid vector with ''Xba''I and ''Spe''I (X sticky ends can come together with S sticky ends), it recircularized without the introduction of our inserts.</p>
 +
*<p style=”text-align:justify;”>Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction.</p>
 +
 
 +
''Marcelo''
 +
 
 +
 
 +
 
 +
==== CeiB: Searching the right colony ====
 +
 
 +
*<p style=”text-align:justify;”>We decided to search in the plate possible cells with the right DNA insert. We made 15 colony of each plate.  Unfortunately we didn’t get it again.</p>
 +
 
 +
''Ane''
 +
 
 +
==''' YeastGuard '''==
 +
 
 +
====Biofusion vector - Electroelution====
 +
 
 +
*<p style=”text-align:justify;”>Considering that we need more vector to perform the ligation reactions, we electroeluted more vector from  the ''ADH1'' biobrick previously digested with ''Xba''I and ''Spe''I ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroelution Protocol 12]).</p>
 +
 
 +
''Taís''
 +
 
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 03:44, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

Getting the visa

  • Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D

finO and finP - Still Trying to Confirm our Biobricks

  • We ran an agarose gel of yesterday's PCRs product.

  • We couldn't obtain even a single amplified fragment! =(

  • Why can the transformed cells grew in the media (that means they actually have the AMP resistence), but they haven't our inserts into it's plasmids?

  • Our advisors suggested us that, since we digested our plasmid vector with XbaI and SpeI (X sticky ends can come together with S sticky ends), it recircularized without the introduction of our inserts.

  • Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction.

Marcelo


CeiB: Searching the right colony

  • We decided to search in the plate possible cells with the right DNA insert. We made 15 colony of each plate. Unfortunately we didn’t get it again.

Ane

YeastGuard

Biofusion vector - Electroelution

  • Considering that we need more vector to perform the ligation reactions, we electroeluted more vector from the ADH1 biobrick previously digested with XbaI and SpeI (Protocol 12).

Taís




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/September_25"