Team:UNICAMP-Brazil/Notebooks/September 5

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''Taís''
''Taís''
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==''' YeastGuard '''==
 
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====Terminator Biobrick====
 
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*We purified the Terminator digestion from agarose gel using Pure Link Kit.
 
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==''' ColiGuard '''==
==''' ColiGuard '''==
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==== Cre-Recombinase ====
==== Cre-Recombinase ====
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* We inoculated four colonies in liquid LB-Amp media in order to make a miniprep tomorrow.  
* We inoculated four colonies in liquid LB-Amp media in order to make a miniprep tomorrow.  
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* We let then grow for overnight at 37oC.   
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* We let then grow for overnight at 37°C.   
''Víctor''
''Víctor''
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====finO and finP Isolation from R Plasmid====
====finO and finP Isolation from R Plasmid====
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* After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed on July 25th, and then we ran an eletrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.
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*<p style=”text-align:justify;”>After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed on July 25th, and then we ran an electrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.</p>
[[Image:isolar_finOP.JPG‎ |center|‎]]
[[Image:isolar_finOP.JPG‎ |center|‎]]
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As shown is the photo, the finO's PCR product reached about 600 bp, and the finP's PCR product reached about 120 bp. The size reached by both products correspond to the estimated lenght of the finO and finP sequences, suggesting that we amplified the correct fragments, and that we sucessful isolated both sequences from the R plasmid.
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*<p style=”text-align:justify;”>As shown is the photo, the finO's PCR product reached about 600 bp, and the finP's PCR product reached about 120 bp. The size reached by both products correspond to the estimated lenght of the finO and finP sequences, suggesting that we amplified the correct fragments, and that we sucessful isolated both sequences from the R plasmid.</p>
''Marcelo''
''Marcelo''
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==''' YeastGuard '''==
 +
 +
====Terminator Biobrick====
 +
 +
*We purified the Terminator digestion from agarose gel using Pure Link Kit ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7]).
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 02:11, 22 October 2009

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MicroGuards

  • We made more 22 LB-AMP plates.

Taís

ColiGuard

Cre-Recombinase

  • The colonies with Cre Recombinase (BBa_J61047) plated yesterday grew up very well!
  • We inoculated four colonies in liquid LB-Amp media in order to make a miniprep tomorrow.
  • We let then grow for overnight at 37°C.

Víctor

finO and finP Isolation from R Plasmid

  • After the successful recovery of the R plasmid (July 24th), we could start working on the isolation of the finO and finP sequences from this plasmid. We performed a PCR using the primers we designed on July 25th, and then we ran an electrophoresis gel with the products, in order to confirm that we indeed amplified the correct fragments.


‎

  • As shown is the photo, the finO's PCR product reached about 600 bp, and the finP's PCR product reached about 120 bp. The size reached by both products correspond to the estimated lenght of the finO and finP sequences, suggesting that we amplified the correct fragments, and that we sucessful isolated both sequences from the R plasmid.

Marcelo

YeastGuard

Terminator Biobrick

  • We purified the Terminator digestion from agarose gel using Pure Link Kit (Protocol 7).





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