Team:UNICAMP-Brazil/Notebooks/August 11

(Difference between revisions)

Latest revision as of 00:34, 22 October 2009

Today we selected 4 colonies of each transformation to confirm it. We inoculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.

We decided that we won´t need biobrick BBa_J04630, because the other two reporters are OK for our constructions.

We're going to need large amounts of DNA, so we inoculated colonies in liquid media in order to repeat the plasmid extractions of the six biobricks we are really going to use.

Taís

@@ Line 7: / Line 7: @@
 ====Selection of transformants====
-Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB medium containing the appropriate antibiotic and let them grow O/N.
+*<p style=”text-align:justify;”>Today we selected 4 colonies of each transformation to confirm it. We inoculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.</p>
 ==''' YeastGuard '''==
-We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.
+====Recovering the biobricks====
-As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010.
+* We decided that we won´t need biobrick BBa_J04630, because the other two reporters are OK for our constructions.
+* We're going to need large amounts of DNA, so we inoculated colonies in liquid media in order to repeat the plasmid extractions of the six biobricks we are really going to use.
-In order to do the miniprep tomorrow, we selected the colonies of each transformation (of the six biobricks) and innoculated them in LB medium containing the appropriate antibiotic to grow O/N.
 ''Taís''
 {{:Team:UNICAMP-Brazil/inc_rodape}}