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EPF-Lausanne/12 October 2009
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==Wet Lab== | ==Wet Lab== | ||
| + | Digestion assay (with Pst1 and SpeI) in order to check the double transformant -KO strand JRG1046. | ||
| + | We checked 3 clones of each double transformant: RO1.1+BB1 and RO2.4+BB1. | ||
| - | + | N.B: RO1.1+BB1 clone #1 culture wasn't pink at all | |
| + | |||
| + | Took image of agarose gel: | ||
| + | [[Image:121009gel_DTjrg1046.JPG|200px|thumb|center]] | ||
| + | <br><br> | ||
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| + | RO1.1+BB1 #3 and 6 are double transformants: | ||
| + | |||
| + | ( cut RO1 --> band of ~900bp, plasmid-900bp | ||
| + | |||
| + | RO2 --> band of 1.8 kb, plasmid - 1.8 kb | ||
| + | |||
| + | BB --> band of ~ 200bp, 400bp, plasmid - 600bp ) | ||
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| + | |||
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| + | Started protocol to clone the "simple" LovTap into an iGEM plasmid: did the PCR on LovTap with primers that contained the iGEM prefix and suffix | ||
| + | |||
| + | ==People in the lab== | ||
| + | |||
| + | Tú, Heidi | ||
<html><center><a href="https://2009.igem.org/EPF-Lausanne/11_October_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a> | <html><center><a href="https://2009.igem.org/EPF-Lausanne/11_October_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a> | ||
Latest revision as of 08:50, 18 October 2009
Contents |
Wet Lab
Digestion assay (with Pst1 and SpeI) in order to check the double transformant -KO strand JRG1046.
We checked 3 clones of each double transformant: RO1.1+BB1 and RO2.4+BB1.
N.B: RO1.1+BB1 clone #1 culture wasn't pink at all
Took image of agarose gel:
RO1.1+BB1 #3 and 6 are double transformants:
( cut RO1 --> band of ~900bp, plasmid-900bp
RO2 --> band of 1.8 kb, plasmid - 1.8 kb
BB --> band of ~ 200bp, 400bp, plasmid - 600bp )
Started protocol to clone the "simple" LovTap into an iGEM plasmid: did the PCR on LovTap with primers that contained the iGEM prefix and suffix
People in the lab
Tú, Heidi
