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EPF-Lausanne/19 October 2009
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==Wet Lab== | ==Wet Lab== | ||
| + | A new '''gel''' was made to assess if the double transformants really weren't (the last gel was a bit fuzzy). 1% 100ml (done yesterday, stayed in TBE buffer overnight), so 190 V for 30 min. The result isn't better than yesterday. | ||
| + | Put RO1.1 and RO2.4 transformants in DH5alpha, RO1.1 or RO2.4 with mutated BB ( ILE427PHE) double transformants in DH5alpha to grow in 5ml LB | ||
| - | + | Colony PCR of : RO1.1 + BB1 mutated clone #3,6 | |
| + | RO2.4+BB1 mutated clone #4,6 | ||
| + | ==People in the lab== | ||
| + | Basile, Gabriela, Tú | ||
Latest revision as of 18:11, 20 October 2009
Contents |
Wet Lab
A new gel was made to assess if the double transformants really weren't (the last gel was a bit fuzzy). 1% 100ml (done yesterday, stayed in TBE buffer overnight), so 190 V for 30 min. The result isn't better than yesterday.
Put RO1.1 and RO2.4 transformants in DH5alpha, RO1.1 or RO2.4 with mutated BB ( ILE427PHE) double transformants in DH5alpha to grow in 5ml LB
Colony PCR of : RO1.1 + BB1 mutated clone #3,6 RO2.4+BB1 mutated clone #4,6
People in the lab
Basile, Gabriela, Tú
