Team:UNICAMP-Brazil/Notebooks/September 28
From 2009.igem.org
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====Minipreps from BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I78016==== | ====Minipreps from BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I78016==== | ||
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*<p style=”text-align:justify;”>Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.</p> | *<p style=”text-align:justify;”>Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.</p> | ||
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====Purification of pTet and double terminator biobricks==== | ====Purification of pTet and double terminator biobricks==== | ||
- | * Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel. | + | *<p style=”text-align:justify;”>Once we digested those biobricks yesterday, we could proceed today to the purification of the target band from an agarose gel.</p> |
- | * We used Invitrogen's | + | *<p style=”text-align:justify;”>We used Invitrogen's Purelink Quick Gel Extraction Kit, following the manufacturer's protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7]), without modifications.</p> |
''Marcelo'' | ''Marcelo'' | ||
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+ | ==== CeaB and CeiB transformation into plasmid [http://partsregistry.org/Part:pSB1A3 pSBA3] ==== | ||
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+ | *<p style=”text-align:justify;”>Today, we dephosphorilated the plasmid [http://partsregistry.org/Part:pSB1A3 pSBA3], and immediately started the ligation reaction of this unphosphorylated plasmid with CeaB and CeiB. Considering that the dephosphorylation use buffer that contains salt and that salt could be interfering in the transformations, we performed a dyalisis to get the samples free of salts. At last, we transformed competent ''E. coli'' (according to the [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation protocol 3]) and plated the respective plates. This time we expect to obtain transforming cells.</p> | ||
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+ | ''Luige'' | ||
==''' YeastGuard '''== | ==''' YeastGuard '''== |
Latest revision as of 03:26, 22 October 2009
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