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EPF-Lausanne/13 October 2009
From 2009.igem.org
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==Wet Lab== | ==Wet Lab== | ||
| - | Clone LOVTAP into | + | |
| - | LOVTAP = PCR product from 12 Oct 09 ( PCR on | + | Clone LOVTAP into an iGEM plasmid: |
| + | |||
| + | LOVTAP = purified PCR product from 12 Oct 09 ( PCR on plasmid with LOVTAP-Term) | ||
| + | |||
Plasmid = pSB1A3 ( Ampicilin resistance) | Plasmid = pSB1A3 ( Ampicilin resistance) | ||
| - | Digestion | + | Digestion (both vector and insert with EcoRI and SpeI) |
| - | + | Checked digestion product with agarose gel | |
Purification | Purification | ||
| Line 38: | Line 41: | ||
Ligation ( 1h30 ) | Ligation ( 1h30 ) | ||
| - | Transformation on LB agar plate | + | Transformation on LB agar plate with ampicilin for selection (+did negative control) |
==People in the lab== | ==People in the lab== | ||
| - | + | Gabriela, Heidi, Tú | |
<html><center><a href="https://2009.igem.org/EPF-Lausanne/12_October_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a> | <html><center><a href="https://2009.igem.org/EPF-Lausanne/12_October_2009"><img src="https://static.igem.org/mediawiki/2009/thumb/6/61/Flèche_gauche.png/70px-Flèche_gauche.png"></a> | ||
Latest revision as of 10:12, 14 October 2009
Contents |
Wet Lab
Clone LOVTAP into an iGEM plasmid:
LOVTAP = purified PCR product from 12 Oct 09 ( PCR on plasmid with LOVTAP-Term)
Plasmid = pSB1A3 ( Ampicilin resistance)
Digestion (both vector and insert with EcoRI and SpeI)
Checked digestion product with agarose gel
Purification
Ligation ( 1h30 )
Transformation on LB agar plate with ampicilin for selection (+did negative control)
People in the lab
Gabriela, Heidi, Tú
