"
EPF-Lausanne/12 October 2009
From 2009.igem.org
(Difference between revisions)
(→Wet Lab) |
(→Wet Lab) |
||
| (4 intermediate revisions not shown) | |||
| Line 27: | Line 27: | ||
==Wet Lab== | ==Wet Lab== | ||
Digestion assay (with Pst1 and SpeI) in order to check the double transformant -KO strand JRG1046. | Digestion assay (with Pst1 and SpeI) in order to check the double transformant -KO strand JRG1046. | ||
| - | We | + | |
| + | We checked 3 clones of each double transformant: RO1.1+BB1 and RO2.4+BB1. | ||
| + | |||
N.B: RO1.1+BB1 clone #1 culture wasn't pink at all | N.B: RO1.1+BB1 clone #1 culture wasn't pink at all | ||
| - | |||
| - | RO1.1+BB1 #3 and 6 are double | + | |
| + | Took image of agarose gel: | ||
| + | [[Image:121009gel_DTjrg1046.JPG|200px|thumb|center]] | ||
| + | <br><br> | ||
| + | |||
| + | RO1.1+BB1 #3 and 6 are double transformants: | ||
| + | |||
( cut RO1 --> band of ~900bp, plasmid-900bp | ( cut RO1 --> band of ~900bp, plasmid-900bp | ||
| + | |||
RO2 --> band of 1.8 kb, plasmid - 1.8 kb | RO2 --> band of 1.8 kb, plasmid - 1.8 kb | ||
| + | |||
BB --> band of ~ 200bp, 400bp, plasmid - 600bp ) | BB --> band of ~ 200bp, 400bp, plasmid - 600bp ) | ||
| - | + | ||
| + | Started protocol to clone the "simple" LovTap into an iGEM plasmid: did the PCR on LovTap with primers that contained the iGEM prefix and suffix | ||
==People in the lab== | ==People in the lab== | ||
Latest revision as of 08:50, 18 October 2009
Contents |
Wet Lab
Digestion assay (with Pst1 and SpeI) in order to check the double transformant -KO strand JRG1046.
We checked 3 clones of each double transformant: RO1.1+BB1 and RO2.4+BB1.
N.B: RO1.1+BB1 clone #1 culture wasn't pink at all
Took image of agarose gel:
RO1.1+BB1 #3 and 6 are double transformants:
( cut RO1 --> band of ~900bp, plasmid-900bp
RO2 --> band of 1.8 kb, plasmid - 1.8 kb
BB --> band of ~ 200bp, 400bp, plasmid - 600bp )
Started protocol to clone the "simple" LovTap into an iGEM plasmid: did the PCR on LovTap with primers that contained the iGEM prefix and suffix
People in the lab
Tú, Heidi
