Team:UNICAMP-Brazil/Notebooks/September 15

(Difference between revisions)

Revision as of 19:21, 17 October 2009

The cultures grew well and were used to perform the miniprep for F plasmid's extraction, according to Protocol 2.
The resulting product of the performed miniprep was applied to an 0,8% agarose gel to verify if it went well. Two bands showed up in the gel, with the largest appearing to be a 23 kb fragment. The lower molecular weight fragment, showing to be approximately a 21 kb fragment, is the supercoiled F plasmid that results from the miniprep protocol.
The next step is to perform the enzymatic digestion of the extracted F plasmid with the HindIII enzyme.

Gabriel

<p style=”text-align:justify;”>We continued our work with finO and finP today by performing minipreps from yesterday's inoculated cultures, according to Protocol 2.
We had 10 cultures for finO and 10 cultures for finP, in order to assure that at least one of them would have the inserted fragment into it's plasmid.

Marcelo

@@ Line 8: / Line 8: @@
 *<p style=”text-align:justify;”>The cultures grew well and were used to perform the miniprep for F plasmid's extraction, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].</p>
+*<p style=”text-align:justify;”>The resulting product of the performed miniprep was applied to an 0,8% agarose gel to verify if it went well. Two bands showed up in the gel, with the largest appearing to be a 23 kb fragment. The lower molecular weight fragment, showing to be approximately a 21 kb fragment, is the supercoiled F plasmid that results from the miniprep protocol.</p>
+*<p style=”text-align:justify;”>The next step is to perform the enzymatic digestion of the extracted F plasmid with the HindIII enzyme.
 ''Gabriel''