Team:UNICAMP-Brazil/Notebooks/September 20

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==''' YeastGuard '''==
==''' YeastGuard '''==
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''Wesley''
''Wesley''
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==''' ColiGuard '''==
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====Cre-REcombinase - transformations results====
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* Today we checked yesterday's plates and, unfortunately, all grown colonies showed a red coloration!
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* That means that the vector probably religated with it's own part (RFP device). This could be avoided by purification of the plasmid's digested band.
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''Víctor''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 18:02, 21 October 2009

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Back to Calendar

YeastGuard

With the product of the mini-prep played yesterday we performed a PCR to verify the correct ligation of the ……… part into the ……. plasmid. In this PCR we used primers that join in the plasmid (VFoward and VReverse) and a primer that join in the insert (…… reverse).

Wesley

ColiGuard

Cre-REcombinase - transformations results

  • Today we checked yesterday's plates and, unfortunately, all grown colonies showed a red coloration!
  • That means that the vector probably religated with it's own part (RFP device). This could be avoided by purification of the plasmid's digested band.

Víctor





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