Team:UNICAMP-Brazil/Notebooks/October 4

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====New strategy: pGEM====
====New strategy: pGEM====
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*<p style=”text-align:justify;”>* Only the plates containing ''E. coli'' transformed with pDLD and Lysozyme showed colonies. Tomorrow we will make colony PCR to collect the right ones.</p>
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*<p style=”text-align:justify;”>Only the plates containing ''E. coli'' transformed with pDLD and Lysozyme showed colonies. Tomorrow we will make colony PCR to collect the right ones.</p>

Revision as of 00:43, 21 October 2009

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ColiGuard

finOP and Cre-Recombinase with pGEM strategy

  • Yesterday's transformed and plated cells grew in the media! We found white and blue colonies in the plate just as expected (due to the beta-galactosidase gene contained in pGEM vector).
  • We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media.
  • Inocula were keep at 37ºC, under 250 rpm, for an O/N period.

Fabi, Leo, Marcelo and Victor

Transformation of the BBa K112806 + BBa B0015 ligation

  • We transformed the ligation made yesterday by eletroporation according to the Protocol 3

Léo


YeastGuard

New strategy: pGEM

  • Only the plates containing E. coli transformed with pDLD and Lysozyme showed colonies. Tomorrow we will make colony PCR to collect the right ones.


Raíssa and Taís




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