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Team:UNICAMP-Brazil/Notebooks/October 14
From 2009.igem.org
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*<p style=”text-align:justify;”>We did miniprep of 10 Adh1+Lysozyme colonies.</p> | *<p style=”text-align:justify;”>We did miniprep of 10 Adh1+Lysozyme colonies.</p> | ||
| - | *<p style=”text-align:justify;”>We digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector ( | + | *<p style=”text-align:justify;”>We digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP358). We used the ''Xba''I and ''Pst''I enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp.</p> |
[[Image:adh1+lis.jpg|500px|center]] | [[Image:adh1+lis.jpg|500px|center]] | ||
| - | *<p style=”text-align:justify;”>We chose two fragments and purified them from the agarose gel. We ligated them to | + | *<p style=”text-align:justify;”>We chose two fragments and purified them from the agarose gel. We ligated them to YEP358. We will transform it tomorrow.</p> |
Revision as of 18:42, 21 October 2009
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