Team:UNICAMP-Brazil/Notebooks/October 9
From 2009.igem.org
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*<p style=”text-align:justify;”>We selected 13 colonies of the transformation we did yesterday to perform a colony-PCR screening. We used 2 pairs of primers:</p> | *<p style=”text-align:justify;”>We selected 13 colonies of the transformation we did yesterday to perform a colony-PCR screening. We used 2 pairs of primers:</p> | ||
- | + | <p style=”text-align:justify;”>-VF/VR, the verification primers of BBa_J23100 plasmid.</p> | |
- | + | <p style=”text-align:justify;”>-Ppy-F-1 and Ppy-R, the pair of primers that amplify our inserted sequence (PY1).</p> | |
*<p style=”text-align:justify;”>We analyzed the results of our PCR in an agarose gel:</p> | *<p style=”text-align:justify;”>We analyzed the results of our PCR in an agarose gel:</p> | ||
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''Fabi and Léo'' | ''Fabi and Léo'' | ||
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==''' YeastGuard '''== | ==''' YeastGuard '''== |
Revision as of 05:32, 21 October 2009
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