Team:UNICAMP-Brazil/Notebooks/August 21

(Difference between revisions)

Latest revision as of 01:00, 22 October 2009

Still fighting for RBS

We digested more plasmids with the SpeI and PstI enzymes (Protocol 14). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.

Raíssa

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 '''Still fighting for RBS'''
-* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digest a little longer and put less amount of DNA into the reaction. This time it seamed to work.
+* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digesting a little longer and put less amount of DNA into the reaction. This time it seamed to work.
 [[image:20090822_Rá_digestao 2 rbs 22_08_09.JPG|150px|center]]