Team:Kyoto/CiC/Notebook/0907-0911

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0907-0911: constructing HIV-TAT-(LALAAAA)3-generator
0914-0918: constructing HIV-TAT-(LALAAAA)3-generator
0921-0925: constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002: constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009: constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016: Observation
1019-1023: Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator

Wednesday, 16 September

To Do

PCR
- HIV-TAT-(LALAAAA)3
Restriction Enzyme Digestion
- HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
- B0015 (EcoR1 + Spe1)
Electrophoresis
- HIV-TAT-(LALAAAA)3
- B0015

Results

ligation

conc.
NO.	Sample name	conc./(ng/ul)
1	11(stu1)	103.2

Transformation

No colony was observed (090917 10:00)　

Thursday, 17 September

To Do

Make repetitive sequence (MPR)
Transformation(retry)

@@ Line 29: / Line 29: @@
 </html>
 {{:Team:Kyoto/CiC/Notebook_tpl}}
-==0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator ==
+==097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator ==
 {{:Team:Kyoto/Pad_start_dates}}
-===Monday, 14 August===
-====To Do====
-*p-2-(1)(4)(7)(8)(10)
-**Miniprep
-**Restriction Enzyme Digestion
-*p-2-(1)(4)  E.X
-**PCR purification kit
-*p-2-(1)(7)(8)(10)  E.S
-**Gel extraction
-*4-(1)(2)(3)
-**Colony PCR
-**Insert check
-====Results====
-=====p-2-(1)(4)(7)(8)(10)=====
-=====p-2-(1)(7)(8)(10)  E.S=====
-*Gel extraction kit
-[[Image:Kyoto_0914_PCR.png|250px]]
-{| class="table"
-|+Miniprep product conc.
-!NO.!!Sample name!!conc./(ng/ul)
-|-
-|1||p-2-(1)||9.3
-|-
-|2||p-2-(7)||25.9
-|-
-|3||p-2-(8)||8.6
-|-
-|4||p-2-(10)||7.5
-|}
-=====4-(1)(2)(3)=====
-[[Image:Kyoto_0914_PCR_2.png|250px]]
-*This result indicates that (1)a, (2)b and (3)a were inserted properly.
-{{:Team:Kyoto/Pad_between_dates}}
-===Tuesday, 15 September===
-====To Do====
-'''Further to 14 Sept.'''
-*p-2-(1)(4)(7)(8)(10)
-**Miniprep
-**Restriction Enzyme Digestion
-*p-2-(1)(4)  E.X
-**PCR purification kit
-*p-2-(1)(7)(8)(10)  E.S
-**Gel extraction
-*4-(1)(2)(3)
-**Colony PCR
-**Insert check
-{{:Team:Kyoto/Pad_between_dates}}
 ===Wednesday, 16 September===
 ====To Do====
-**ligation
+*PCR
-**transformation
+**HIV-TAT-(LALAAAA)3
+*Restriction Enzyme Digestion
+**HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
+**B0015 (EcoR1 + Spe1)
+*Electrophoresis
+**HIV-TAT-(LALAAAA)3
+**B0015
 ====Results====
 =====ligation=====