Team:Kyoto/CiC/Notebook/0907-0911

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(Difference between revisions)
(→0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator)
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**B0015 (EcoR1 + Spe1)
**B0015 (EcoR1 + Spe1)
*Electrophoresis
*Electrophoresis
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**HIV-TAT-(LALAAAA)3
+
 
-
**B0015
+
====Results====
====Results====
=====ligation=====
=====ligation=====

Revision as of 09:44, 21 October 2009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator

Wednesday, 16 September

To Do

  • PCR
    • HIV-TAT-(LALAAAA)3
  • Restriction Enzyme Digestion
    • HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
    • B0015 (EcoR1 + Spe1)
  • Electrophoresis

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00)　

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0907-0911"