Team:Kyoto/CiC/Notebook/0907-0911

From 2009.igem.org

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====Results====
====Results====
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=====ligation=====
 
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{| class="table"
 
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|+conc.
 
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!NO.!!Sample name!!conc./(ng/ul)
 
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|-
 
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|1||11(stu1)||103.2
 
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|}
 
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=====Transformation=====
 
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No colony was observed (090917 10:00)　
 
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{{:Team:Kyoto/Pad_between_dates}}
 
===Thursday, 17 September===
===Thursday, 17 September===

Revision as of 09:45, 21 October 2009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator

Wednesday, 16 September

To Do

  • PCR
    • HIV-TAT-(LALAAAA)3
  • Restriction Enzyme Digestion
    • HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
    • B0015 (EcoR1 + Spe1)
  • Electrophoresis

Results

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0907-0911"