Team:Kyoto/CiC/Notebook/0921-0925

From 2009.igem.org

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(Monday, 14 August)
(Tuesday, 15 September)
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===Tuesday, 15 September===
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===Tuesday, 21 September===
====To Do====
====To Do====
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'''Further to 14 Sept.'''
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*HIV-TAT-(LALAAAA)3-generator
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*p-2-(1)(4)(7)(8)(10)
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**Miniprep
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**Restriction Enzyme Digestion
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*p-2-(1)(4)  E.X
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**PCR purification kit
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*p-2-(1)(7)(8)(10)  E.S
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**Gel extraction
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*4-(1)(2)(3)
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**Colony PCR
**Colony PCR
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**Insert check
 
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===Wednesday, 16 September===
===Wednesday, 16 September===
====To Do====
====To Do====

Revision as of 11:38, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.


Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • 消えた



Tuesday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR



Wednesday, 16 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)