IBB Pune/25 June 2009

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I)
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DISCUSSIONS WITH PRAVEEN SIR...
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*Ligation needs to be practised.
 +
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*PCR-ligation-Transformation takes time to come. no colonies even after 16 hours.
 +
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*Troubleshooting,
 +
:*Kan too High? Try lowering KAn.
 +
:*Ligation, try blunt end with the cohesive
 +
:*Is PCR the right way to manna? Try transformation.
 +
:*Proceed with the current thing, wait patiently for colonies to appear.
 +
 +
II)Plasmid isolation started from single colony from P4N.

Latest revision as of 14:14, 21 October 2009




I) DISCUSSIONS WITH PRAVEEN SIR...

  • Ligation needs to be practised.
  • PCR-ligation-Transformation takes time to come. no colonies even after 16 hours.
  • Troubleshooting,
  • Kan too High? Try lowering KAn.
  • Ligation, try blunt end with the cohesive
  • Is PCR the right way to manna? Try transformation.
  • Proceed with the current thing, wait patiently for colonies to appear.

II)Plasmid isolation started from single colony from P4N.

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