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Overview Sensitivity Tuner --- Characterisation --- Modelling Colour Generators --- Carotenoids (Orange/Red) --- Melanin (Brown) --- Violacein (Purple/Green) The Future Safety

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Pigments

The Kit of Parts

Though E. coli does not naturally produce pigment, several other bacterial species secrete pigmented antibiotics. We mined bacterial genomes for pigment-producing operons, and transformed the most prominent candidates into E. coli. In particular, we have devoted our summer to 3 different pigment systems:

• Carotenoids: The enzymes required for carotenoid production originally come from Pantoea ananatis, and were available in the registry. We used them to produce orange and red.
• Melanin: The tyrosinase required for melanin production originally comes from Rhizobium etli and produces brown.
• Violacein: The enzymes required for voilacein production originally come from Chromobacterium violacein. The operon can be manipulated to produce voilet, green, and blue.

As as a result of our efforts, this summer, we have submitted the following biobricks to the Registry. We hope that in the future this this kit of parts will be expanded in the future.

TABLE OF PIGMENT BIOBRICKS