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Team:UNICAMP-Brazil/Notebooks/September 2
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====Lysozyme PCR: ''Gallus gallus'' template==== | ====Lysozyme PCR: ''Gallus gallus'' template==== | ||
| - | The results obtained today were extremely awaited. | + | *<p style=”text-align:justify;”>The results obtained today were extremely awaited. We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs (1: pDN6; 2: oligoDT) and the RNA without treatment (because there was still genomic in it!).</p> |
| - | We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs (1: pDN6; 2: oligoDT) and the RNA without treatment (because there was still genomic in it!). | + | |
[[image:20090902_PCR_Lysozyma_só_ela.JPG|250px|center]] | [[image:20090902_PCR_Lysozyma_só_ela.JPG|250px|center]] | ||
| - | As you can see in the gel, we got specific bands from the oviduct sample! =] | + | *<p style=”text-align:justify;”>As you can see in the gel, we got specific bands from the oviduct sample! =]</p> |
''Taís'' | ''Taís'' | ||
====RBS+CFP ligation==== | ====RBS+CFP ligation==== | ||
| - | We verified using electrophoresis, the results of the digestion made yesterday (RBS+CFP plasmids). The gel showed we had no success with the digestions. | + | *<p style=”text-align:justify;”>We verified using electrophoresis, the results of the digestion made yesterday (RBS+CFP plasmids). The gel showed we had no success with the digestions.</p> |
''Wesley'' | ''Wesley'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 02:05, 22 October 2009
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