Team:UNICAMP-Brazil/Notebooks/October 14
From 2009.igem.org
(→PY Promoter - Preparation of electrocompetent cells of the conjugative strain) |
(→PY Promoter - Preparation of electrocompetent cells of the conjugative strain) |
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==== PY Promoter - Preparation of electrocompetent cells of the conjugative strain ==== | ==== PY Promoter - Preparation of electrocompetent cells of the conjugative strain ==== | ||
- | *<p style=”text-align:justify;”>Now that we have PY1 promoter + RFP inserted in a plasmid with | + | *<p style=”text-align:justify;”>Now that we have PY1 promoter + RFP inserted in a plasmid with kanamycin resistance we are able to transform the conjugative strain. As we said before, this conjugative strain already has ampicilin resistance, that is why we inserted our construction in a plasmid with a different resistance.</p> |
*<p style=”text-align:justify;”>Before transforming this strain we need to prepair electrocompetent cells. So today we inoculated a colony of this strain in liquid LB-AMP at 37ºC to prepare the competent cells tomorrow.</p> | *<p style=”text-align:justify;”>Before transforming this strain we need to prepair electrocompetent cells. So today we inoculated a colony of this strain in liquid LB-AMP at 37ºC to prepare the competent cells tomorrow.</p> |
Revision as of 19:28, 21 October 2009
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