Team:UNICAMP-Brazil/Notebooks/October 19
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====Yeast experiments==== | ====Yeast experiments==== | ||
- | *<p style=”text-align:justify;”>There are plenty of yeast colonies in the pDLD+Lys and | + | *<p style=”text-align:justify;”>There are plenty of yeast colonies in the pDLD+Lys and ''ADH1''+Lys plates. =)</p> |
- | *<p style=”text-align:justify;”>We did PCR with 30 yeast colonies transformed with | + | *<p style=”text-align:justify;”>We did PCR with 30 yeast colonies transformed with ''ADH1''+Lysozyme and pDLD+Lysozyme. Unfortunately none of the colonies had the correct vector =/</p> |
[[Image:adh1+lis2.jpg|400px|center]] | [[Image:adh1+lis2.jpg|400px|center]] | ||
[[Image:pDLD+lis.jpg|500px|center]] | [[Image:pDLD+lis.jpg|500px|center]] | ||
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*<p style=”text-align:justify;”>We have no time to repeat the transformations =( Our hope is that there has been a problem to the PCR reaction and that the parts are correctly connected to the YEP vector. So we decided to go on with the transformed yeasts we have.</p> | *<p style=”text-align:justify;”>We have no time to repeat the transformations =( Our hope is that there has been a problem to the PCR reaction and that the parts are correctly connected to the YEP vector. So we decided to go on with the transformed yeasts we have.</p> | ||
- | *<p style=”text-align:justify;”>At night we find plenty yeast colonies in the pDLD+YFP and | + | *<p style=”text-align:justify;”>At night we find plenty yeast colonies in the pDLD+YFP and ''ADH1''+YFP plates.</p> |
- | *<p style=”text-align:justify;”>Considering our lack of time we decided to collect a pool of yeast colonies for each construction and inoculate in liquid media. This strategy was adopted for both constructions (pDLD+YFP and | + | *<p style=”text-align:justify;”>Considering our lack of time we decided to collect a pool of yeast colonies for each construction and inoculate in liquid media. This strategy was adopted for both constructions (pDLD+YFP and ''ADH1''+YFP). These pre inocula will be used for induction tests of Lysozyme tomorrow. |
Latest revision as of 03:37, 22 October 2009
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