Team:UNICAMP-Brazil/Notebooks/August 11

From 2009.igem.org

(Difference between revisions)
(→ColiGuard)
(→YeastGuard)
 
Line 13: Line 13:
====Recovering the biobricks====
====Recovering the biobricks====
-
* We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.
+
* We decided that we won´t need biobrick BBa_J04630, because the other two reporters are OK for our constructions.
-
* We will need large amounts of DNA so we inoculated colonies in liquid media in order to repeated the plasmid extractions of the six biobricks we are really going to use.
+
* We're going to need large amounts of DNA, so we inoculated colonies in liquid media in order to repeat the plasmid extractions of the six biobricks we are really going to use.

Latest revision as of 00:34, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

Selection of transformants

  • Today we selected 4 colonies of each transformation to confirm it. We inoculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.

YeastGuard

Recovering the biobricks

  • We decided that we won´t need biobrick BBa_J04630, because the other two reporters are OK for our constructions.
  • We're going to need large amounts of DNA, so we inoculated colonies in liquid media in order to repeat the plasmid extractions of the six biobricks we are really going to use.


Taís




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_11"