Team:UNICAMP-Brazil/Notebooks/August 19

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==''' ColiGuard '''==
==''' ColiGuard '''==
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====Enzime restriction====
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====Enzyme restriction====
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Today, we performed two enzyme restrictions of two RBSs recovered in order to prove the transformations. We got it!!!
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*<p style=”text-align:justify;”>Today, we performed two enzymatic digestions of two RBS, recovered in order to prove the transformations. We got it!!!
''Luige''
''Luige''

Latest revision as of 01:10, 22 October 2009

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YeastGuard

Recovering the biobricks

  • In the LB-AMP plates we managed to get isolated colonies, but the LB-KAN plates were the same as the first we had. We think that the antibiotic is not working on these LB-KAN plates, since the supposed antibiotic for the terminator vector is ampicilin.

  • We selected colonies from the LB-AMP plates and inoculated in LB liquid media for the miniprep tomorrow.

  • We also inoculated colonies from the RBS plate for the miniprep.

Taís

ColiGuard

Enzyme restriction

  • Today, we performed two enzymatic digestions of two RBS, recovered in order to prove the transformations. We got it!!!

Luige





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