Team:UNICAMP-Brazil/Notebooks/August 28

From 2009.igem.org

(Difference between revisions)
(→YeastGuard)
(→YeastGuard)
 
Line 14: Line 14:
====Terminator biobrick====
====Terminator biobrick====
-
* Colony PCR
+
*<p style=”text-align:justify;”>Colony PCR</p>
The colony PCR was made to confirm the presence of terminator transformants, because  the antibiotic resistence is not working to any antibiotics.  
The colony PCR was made to confirm the presence of terminator transformants, because  the antibiotic resistence is not working to any antibiotics.  
Line 20: Line 20:
-
We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denatured this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR.
+
We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O. Then, denatured these cells at 95°C during 5 minutes and heat shocked on ice for 5 minutes. Spinned the tubes and used 1 µl to make the PCR.
-
* Preparing for Miniprep
+
*<p style=”text-align:justify;”>Preparing for Miniprep</p>
-
We also inoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow.
+
We also inoculated 10 colonies transformed with the terminator biobrick to perform the miniprep tomorrow.
''Wesley''
''Wesley''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 01:50, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

Kluyveromices lactis genes

  • The PCR for the K. lactis genes worked very well! =] We got specific bands for the 3 sequences (agarose gel above).

Terminator biobrick

  • Colony PCR

The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics.

PCRKL.jpg


We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O. Then, denatured these cells at 95°C during 5 minutes and heat shocked on ice for 5 minutes. Spinned the tubes and used 1 µl to make the PCR.

  • Preparing for Miniprep

We also inoculated 10 colonies transformed with the terminator biobrick to perform the miniprep tomorrow.

Wesley




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_28"