Team:UNICAMP-Brazil/Notebooks/September 18
From 2009.igem.org
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====Cre-Recombinase and pSB1A3 digestion==== | ====Cre-Recombinase and pSB1A3 digestion==== | ||
- | *<p style=”text-align:justify;”>Today we digested the purified PCR product for Cre-Recombinase without ATG start codon (see Septermber 13th) with | + | *<p style=”text-align:justify;”>Today we digested the purified PCR product for Cre-Recombinase without ATG start codon (see Septermber 13th) with ''Xba''I and ''Spe''I restriction enzymes. We also digested the vector pSB1A3 with the same enzymes, aiming in the future ligation between them.</p> |
*<p style=”text-align:justify;”>Digestion lasted 3 hours on both cases. | *<p style=”text-align:justify;”>Digestion lasted 3 hours on both cases. | ||
Revision as of 02:45, 22 October 2009
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