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EPF-Lausanne/25 August 2009
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==Wet Lab== | ==Wet Lab== | ||
| + | |||
| + | ===Miniprep:=== | ||
| + | Made according to the protocol of Aisgen Miniprep Kit Elution in 50 ul. | ||
| + | The RD2 #5 plasmids prep as some that have already been prepared will be sent for sequencing this afternoon. | ||
| + | |||
| + | |||
| + | ===Mediums=== | ||
| + | New medium were made to grow the double-transformed DH5 a (LovTap BB and RO2) | ||
| + | -> should be without thy. | ||
| + | |||
| + | M9 was redone, pH adjusted with amino acid and filtred. | ||
| + | for 1L: | ||
| + | : 5g glucose | ||
| + | : 6g Na2PO4 | ||
| + | : 3g KH2PO4 | ||
| + | : 1g NH4Cl | ||
| + | : 0.5g NaCl | ||
| + | : 0.12g MgSO4 | ||
| + | : 0.01g CaCl2 | ||
| + | |||
| + | |||
| + | ===Trp Operon synthesis=== | ||
| + | Using the Klenow fragments protocol and primers:£ | ||
| + | : Trp operon Rev | ||
| + | : Trp operon Fwd | ||
| + | |||
Revision as of 10:51, 2 September 2009
Wet Lab
Miniprep:
Made according to the protocol of Aisgen Miniprep Kit Elution in 50 ul. The RD2 #5 plasmids prep as some that have already been prepared will be sent for sequencing this afternoon.
Mediums
New medium were made to grow the double-transformed DH5 a (LovTap BB and RO2) -> should be without thy.
M9 was redone, pH adjusted with amino acid and filtred. for 1L:
- 5g glucose
- 6g Na2PO4
- 3g KH2PO4
- 1g NH4Cl
- 0.5g NaCl
- 0.12g MgSO4
- 0.01g CaCl2
Trp Operon synthesis
Using the Klenow fragments protocol and primers:£
- Trp operon Rev
- Trp operon Fwd
People in the lab
Nath, Basile, Gab, Christian
