Team:UNICAMP-Brazil/Notebooks/August 11

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==Coliguard: Selection of transformants==
==Coliguard: Selection of transformants==
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Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB media containg the appropriate antibiotic and let them grow O/N.  
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Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB media containing the appropriate antibiotic and let them grow O/N.  
==Yeastguard==
==Yeastguard==
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As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010.
As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010.
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In order to do the mini-prep tomorrow, we got the colonies from the plates (of the six biobricks) and put them to grow in liquid medium.  
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In order to do the mini-prep tomorrow, we selected the colonies of each transformation (of the six biobricks) and innoculated them in LB media containing the appropriate antibiotic to grow O/N.  
'''Taís'''
'''Taís'''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 20:03, 14 August 2009

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Coliguard: Selection of transformants

Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB media containing the appropriate antibiotic and let them grow O/N.

Yeastguard

We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.

As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010.

In order to do the mini-prep tomorrow, we selected the colonies of each transformation (of the six biobricks) and innoculated them in LB media containing the appropriate antibiotic to grow O/N.

Taís




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