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Team:UNICAMP-Brazil/Notebooks/August 11
From 2009.igem.org
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{{:Team:UNICAMP-Brazil/inc_topo}} | {{:Team:UNICAMP-Brazil/inc_topo}} | ||
{{:Team:UNICAMP-Brazil/inc calendar}} | {{:Team:UNICAMP-Brazil/inc calendar}} | ||
| + | __NOTOC__ | ||
| - | == | + | ==''' ColiGuard '''== |
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| + | ====Selection of transformants==== | ||
Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB media containing the appropriate antibiotic and let them grow O/N. | Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB media containing the appropriate antibiotic and let them grow O/N. | ||
| - | == | + | ==''' YeastGuard '''== |
We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions. | We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions. | ||
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As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010. | As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010. | ||
| - | In order to do the | + | In order to do the miniprep tomorrow, we selected the colonies of each transformation (of the six biobricks) and innoculated them in LB media containing the appropriate antibiotic to grow O/N. |
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| + | ''Taís'' | ||
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{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 18:34, 7 September 2009
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