Team:UNICAMP-Brazil/Notebooks/August 6

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== YeastGuard - Selection of Biobricks ==
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== YeastGuard - Recovering the Biobricks ==
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- Today we chose 7 biobricks to the yeastguard project and resuspended them.
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- Today we resuspended the 7 biobricks of the yeastguard project.
- We transformed the biobricks into ''E. coli'' DH10B competent cells and plated on Kan or Amp plates, according to information from registry page.  
- We transformed the biobricks into ''E. coli'' DH10B competent cells and plated on Kan or Amp plates, according to information from registry page.  

Revision as of 20:48, 14 August 2009

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YeastGuard - Recovering the Biobricks

- Today we resuspended the 7 biobricks of the yeastguard project.

- We transformed the biobricks into E. coli DH10B competent cells and plated on Kan or Amp plates, according to information from registry page.

- Prayed to find colonies tomorrow. =)

- Chosen Biobricks: BBa_J63005, BBa_J63003, BBa_J63002, BBa_E2030, BBa_E2020, BBa_J04630 and BBa_J63010.

Raíssa and Wesley

YeastGuard - RNA Extraction

- Today we performed RNA extration from yeast Kluyveromyces lactis according to protocol 6 (see protocol section).




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