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Team:Warsaw/Calendar-Main/22 August 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3><div style="text-align: center;">Assembly of endosomal detection operon</div></h3> <h4>Marcin</h4> <br/> <p>Task 1:</p> <ul> <li>E...) |
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<p>Task 1:</p> | <p>Task 1:</p> | ||
<ul> | <ul> | ||
| - | <li>Electrophoresis of plasmid isolated and subsequently digested in: <a href="https://2009.igem.org/Team:Warsaw/Calendar-Main/ | + | <li>Electrophoresis of plasmid isolated and subsequently digested in: <a href="https://2009.igem.org/Team:Warsaw/Calendar-Main/21_August_2009">21.08.09</a></li></ul> |
<p>Results:</p> | <p>Results:</p> | ||
<p>All samples of cro CDS on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> are without insert</p> | <p>All samples of cro CDS on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> are without insert</p> | ||
<p><b>Comment:</p></b> | <p><b>Comment:</p></b> | ||
<p>The most probable explanation of this situation is circularization of empty plasmid because I have forgotten to dephosphorylate vector using CIAP</p> | <p>The most probable explanation of this situation is circularization of empty plasmid because I have forgotten to dephosphorylate vector using CIAP</p> | ||
| + | <br/> | ||
<p>Task 2:</p><ul><li>Prepare the bacterial cultures for isolation of following construct: <a href="http://partsregistry.org/Part:BBa_C0040"><span style="color: black">BBa_C0040</a></span> with <a href="http://partsregistry.org/Part:BBa_B0032"><span style="color: black">BBa_B0032</a></span> + <a href="http://partsregistry.org/Part:BBa_R0080"><span style="color: black">BBa_R0080</a></span> on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li></ul> | <p>Task 2:</p><ul><li>Prepare the bacterial cultures for isolation of following construct: <a href="http://partsregistry.org/Part:BBa_C0040"><span style="color: black">BBa_C0040</a></span> with <a href="http://partsregistry.org/Part:BBa_B0032"><span style="color: black">BBa_B0032</a></span> + <a href="http://partsregistry.org/Part:BBa_R0080"><span style="color: black">BBa_R0080</a></span> on <a href="http://partsregistry.org/Part:pSB1A3"><span style="color: black">pSB1A3</a></span> plasmid</li></ul> | ||
Revision as of 05:58, 25 August 2009
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Assembly of endosomal detection operon
Marcin
Task 1:
- Electrophoresis of plasmid isolated and subsequently digested in: 21.08.09
Results:
All samples of cro CDS on pSB1A3 are without insert
Comment:
The most probable explanation of this situation is circularization of empty plasmid because I have forgotten to dephosphorylate vector using CIAP
Task 2:
- Prepare the bacterial cultures for isolation of following construct: BBa_C0040 with BBa_B0032 + BBa_R0080 on pSB1A3 plasmid
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